Distribution of human papillomavirus in precancerous and cancerous cervical neoplasia in Tunisian women
نویسندگان
چکیده
Abstract Background High-risk human papillomavirus (HR-HPV) are responsible for cervical cancer (CC) which represents the second most prevalent gynecological among Tunisian women. Preventive strategies against CC based on prophylactic vaccines that have not yet been implemented into national vaccination program of Tunisia. Therefore, present study aimed to investigate HPV genotypes distribution in neoplasia women order predict impact using current prevention Methods A total 200 formalin-fixed paraffin embedded biopsies were collected our study. DNA was extracted Qiagen Mini prep kit. quality controlled by Beta Globin PCR. Only positive samples test used. detection performed a nested PCR PYGMY and GP5+/6+ primers. Genotyping Reverse Line hybridization 31 probes. Results The mean age participants 38.97 years 75% over 30 years. Cervical according showed CINII/CINIII observed old. All Overall prevalence lesions 83% (166/200). 65% CINI, 82% 85% CC. HR-HPV statistically significantly associated with intraepithelial (p < 10 –3 ). lesion grade HPV16 HPV18 all lesions. For CINII/CINIII, 35 (37.5%) detected type, followed (33.3%) 45 (28.5%) 16 (18.9%). 45(57.5%), 18 (53.3%) HPV58, 59, 68 only HPV45, HPV16. HPV39, 31, 33, 52, 56 HPV70 CINI. Conclusions Our findings can give useful information vaccine implementation helping health policymakers choose appropriate type
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ژورنال
عنوان ژورنال: Infectious Agents and Cancer
سال: 2021
ISSN: ['1750-9378']
DOI: https://doi.org/10.1186/s13027-021-00392-1